Staff from six branches of Lloyds Bank aim to raise £4,000 for the John Eastwood Hospice, Sutton.
They will each be walking 367,000 steps to represent how many people are diagnosed with cancer each year in the UK.
Around 75 members of staff hope to raise at least £2,000 for the hospice, which Lloyds Bank will match.
The six Lloyds branches taking part are Mansfield, Kirkby, New Ollerton, Shirebrook, Sutton and Worksop.
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